Prednisone is a synthetic corticosteroid functioning as a glucocorticoid receptor agonist. Supplied as lyophilized powder, Prednisone is utilized in preclinical research examining glucocorticoid receptor signaling, immunosuppressive mechanisms, and steroid hormone pharmacology in cellular and tissue models investigating inflammation and immune function.

Chemical/Compound Information:

• Chemical Name: 17,21-Dihydroxypregna-1,4-diene-3,11,20-trione
• Molecular Formula: C21H26O5
• Molecular Weight: 358.43 g/mol
• Compound Class: Synthetic Corticosteroid / Glucocorticoid Receptor Agonist

Prednisone serves as a prodrug activated by hepatic 11β-hydroxysteroid dehydrogenase to form the active metabolite prednisolone. Investigation of prednisone enables systematic exploration of glucocorticoid receptor-mediated gene transcription, anti-inflammatory signaling cascades, and steroid hormone pharmacodynamics in preclinical immunology models.

Research Applications:

• Glucocorticoid receptor (GR) ligand-binding domain interactions and transactivation kinetics
• GR-mediated inflammatory gene suppression in TNF-α or LPS-stimulated cell models
• Immune cell differentiation effects: T regulatory cell induction vs. effector T cell suppression
• Glucocorticoid-induced apoptosis and cell cycle arrest in lymphocyte populations
• Dose-dependent GR occupancy and transcriptional response characterization

Storage and Handling:

• Store at 2-8°C in sealed vial protected from light exposure
• Minimize humidity; include desiccant in storage container
• Reconstitute in DMSO or ethanol for cell-based assays; use PBS for biochemical assays
• Prepare working solutions immediately before experiments
• Protect from direct sunlight throughout all handling steps

Product Specifications:

• Purity: ≥99% (HPLC)
• Appearance: White to off-white lyophilized powder
• Form: Lyophilized powder
• Quantity: As specified per vial labeling

FOR RESEARCH USE ONLY. Not intended for human consumption or clinical use. All products are sold strictly for laboratory, educational, and scientific research purposes.