NAD+ (Nicotinamide Adenine Dinucleotide) is a fundamental coenzyme participating in hundreds of enzymatic reactions across cellular metabolism. Supplied as lyophilized powder, NAD+ is essential for sirtuin pathway research, investigating NAD+-dependent enzymatic mechanisms, and characterizing metabolic regulation through cofactor availability.

Chemical/Compound Information:

• Chemical Name: Nicotinamide Adenine Dinucleotide (Oxidized form)
• Molecular Formula: C21H28N7O14P2
• Molecular Weight: 663.43 g/mol
• Compound Class: Cellular Coenzyme / Nucleotide

NAD+ serves as electron carrier in redox reactions and as substrate for NAD+-consuming enzymes including sirtuins (SIRT1-7), PARPs, and CD38. Investigation of NAD+-dependent pathways enables elucidation of metabolic regulation, cellular stress responses, and translational models of metabolic aging.

Research Applications:

• Sirtuin enzyme catalytic activity and NAD+ substrate requirement characterization
• SIRT1-mediated deacetylation of metabolic enzymes (FOXO, PGC-1α, AMPK)
• NAD+/NADH ratio modulation and redox balance assessment in cell metabolism
• Glycolysis and TCA cycle NAD+-dependent enzymatic flux analysis
• Mitochondrial bioenergetics and electron transport chain NAD+ recycling capacity

Storage and Handling:

• Store at -20°C in sealed vial protected from light exposure
• Minimize freeze-thaw cycles; prepare single-use aliquots from master stock
• Reconstitute in sterile water or appropriate buffer immediately before use
• Avoid repeated exposure to air; maintain inert (N2) headspace in storage vials
• Verify pH stability in reconstituted solutions; use within 4 hours of preparation

Product Specifications:

• Purity: ≥98% (HPLC)
• Appearance: White to off-white lyophilized powder
• Form: Lyophilized powder
• Quantity: 250mg per vial

FOR RESEARCH USE ONLY. Not intended for human consumption or clinical use. All products are sold strictly for laboratory, educational, and scientific research purposes.